Read & analyse. HTRF Signal + 2 hours @ RT [Cortisol] ng/ml 10 µL Standard or Sample 5 µL Cortisol acceptor reagent 5 µL Cortisol donor antibody ADD READ & ANALYSESafire 2™ readers must be appropriately be configured for HTRF readout by setting up the measurement conditions in the “multilabeling” function of Magellan software. Lyse cells Read on an HTRF compatible reader . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Each well generally requires 50µL of lysis buffer 1X. Certified HTRF compatible reader by CisBio Bioassays;Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. For more information about set-up and compatible HTRF ® readers,• HTRF®-Certified Reader. VICTOR® Nivo™ multimode plate reader 2 | For research use only. Incubate 2h at RT Transfer 16 µL supernatant. Neocarzinostatin dose-response on Jurkat cells. It fits any benchtop – and budget Building on our pioneering family of benchtop multimode plate readers, the VICTOR® Nivo™ multimode reader breaks new ground, packing all popular detection modes into the industry’s smallest benchtop. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Seal the plate and incubate 3 hours at RT . Plate 25 μL of cells in. The plate map included a low and a high level calibrator, conjugate blanks, and a specific control to assess the counting rate at 620 nm (i. • HTRF®-Certified Reader. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate Antibody• HTRF®-Certified Reader. PHERAstar FSX Powerful and most sensitive HTS plate reader . As shown in Fig. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. Table 1. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Make sure the setup for Tb Cryptate is used. The new ligands are divided into four groups according to their chemical structures and/or interactions with YTHDC1 (Table 1 and Fig. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. * For HTRF microplate recommendations, please visit binding of the fluorescent ligand to the receptor, TR-FRET occurs and is measured with an HTRF-certified microplate reader. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. Make sure the setup for Eu 3+ Cryptate is used. The plate was covered and incubated for two hours at room temperature. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). (donor) of compounds are measured on an HTRF-compatible reader. HTRF is a proprietary time-resolved fluorescence technology that overcomes Results summary for HTRF IP1 standard curve. Sensitive, accurate and reliable detection is critical across cell, protein and genomic analysis applications in drug discovery and development. Optimized instrument settings for the HTRF camps HiRange assay. A gradient of signal is apparent prior to plate reader optimization (a) causing a %CV of 9. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. **For more information about HTRF® compatible readers and for set-up recommendations, please visit our website at:ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. -Certified Reader. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Data Analysis HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Assay setup for a 384-well low-volume plate. HTRF KinEASE-TK kit and HTRF 96-well low volume plate were obtained from Cisbio Bioassays. The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. Cells in each image are identified by SoftMax Pro Software and cell-by-cell HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF Ratio Anti-Tag Antibody Cryptate Inhibitor-Red Inhibitor Purified Tagged Kinase Figure 2: Principle of HTRF kinase competition binding assay (IC 50 - KI determination) HTRF Dasatinib-Red Part # 62KB02REDC & 62KB02REDE Amount: 1 nmol (62KB02REDC) & 20 nmol (62KB02REDE) Concentration: 25 µM in DMSO Form:. The assay was prepared in both white and black plates and incubated >18 hours. Make sure the setup for Tb Cryptate is used. Alpha Technology: The VICTOR Nivo system. Read other about this measurement approach here. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/vis spectrometer, AAS System, and dedicated AlphaScreen ® and. These convert photons generated by fluorescence or. 68. Incubate overnight at RT. This protocol. The detection antibody is incubated for 1. PLATE READER INTRODUCTION Selecting the right plate reader for your lab can be challenging. Eu cryptate emission level). $1,024. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Its. com HTRF Microplate Readers. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. FOR HTRF CERTIFIED READER For. For more. + 4 µL HTRF pre-mixed antibodies . The kit includes everything you need: reagents, buffers and a microplate. FOR HTRF CERTIFIED READER . Unlike fluorescence intensity which focuses on the quantification of emission intensity at a specific wavelength and neglects its polarization, fluorescence polarization specifically analyses as output the emission intensity of. At Cisbio we provide microplates that help you discover better. Plate 25 μL of cells in. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of total-STING by HTRF. For a list of HTRF-compatible readers and set-up recommendations, please visit . FOR HTRF CERTIFIED READER. For more. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Incubate overnight at RT. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. and Canaa -8-963-4567. It measures at any wavelength, with top and bottom reading for most standard technologies. FOR HTRF CERTIFIED READER. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Its. 96-well culture plate+ 4 µL HTRF pre-mixed antibodies. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. FOR HTRF CERTIFIED READER. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Small ONE- PLATE ASSAY PROTOCOL: overnight FOR HTRF CERTIFIED READER . The SpectraMax i3x and SpectraMax iD5 readers can be equipped with an HTRF-certified detection cartridge (i3x) or an HTRF-certified module and filters (iD5) to meet the demanding specifications required for HTRF assays. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate AntibodyCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. HTRF® Europium cryptate donor / Red acceptor readout Setup recommendations for Tristar 5FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. When MikroWin opens, if you already have a pre-existing template for. If not, our scientific team will help you choose the instrument that best suits your needs and budget. 1. Endpoint mode experiments measure each well of the microplate only once. Step 5 Remove the plate sealer and read on an HTRF® compatible reader The blank control is used to check the Cryptate signal at 620 nm. Certified for use with Alpha, LANCE ® or DELFIA ®. NC1656382. HTRF Microplate Readers. The FLUOstar ® Omega is a multi-mode microplate reader with three detection modes. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. Figure 1. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Detection plates from providers not listed on this. 14 seconds for a 384-well plate. Handle complex biological matrices easily. Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. Read & analyse. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. If not, our scientific team will help you choose the instrument that best suits your needs and budget. • HTRF®-Certified Reader **. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. For more information about HTRF. Manufacturer. Incubate 4h at RT. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. The reader only allows high performance for the used HTRF measurement when the HTRF is run with white plates. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Synergy NEO2 readers must be appropriately configured for HTRF® readout by setting up the measurement conditions in the Gen5™ Reader Control and Data Analysis Software. Reagents were dispensed following Cisbio’s ligand binding protocol (Figure 3). The mean and standard deviation. Read & analyse. The VICTOR Nivo is a HTRF certified microplate reader. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. For more ® information about HTRF compatible readers and for set-up recommendations. 96-well culture plateHowever, time-resolved fluorescence (TRF & TR-FRET) assays are used in conjunction with fluorophores that have a longer half-life. Transfer 5 µL of cell supernatant from the 96-well plate to a 384-small volume (SV) white detection plate . These plates can be read on all HTRF certified readers, thus making their implementation in. Remove the plate seal and read on an HTRF compatible reader. This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. PerkinElmer. 96-well or 384-well small volume (SV) detection microplates - For more information about microplate recommendations, please. PerkinElmer EnVision Multimode Plate Reader. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key features BMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. The ratio of the two fluorescence Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. The plate was covered both incubated for one hour at room temperature. For more information. HTRF certified . In particular, these parameters should be entered as defined in the table below Setup Top filter cube EX 330 / LUM (block #18) EM 620 / 665 / LUM (block #41) Light source LaserDetect flash, glow, Dual Luciferase ® Reporter and BRET assays. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. FOR HTRF CERTIFIED READER. Partnerships for delivering turn-key solutions. + 4 µL HTRF pre-mixed antibodies. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. $352. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Conclusion. FOR HTRF CERTIFIED READER For more information about HTRF. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The VICTOR Nivo is a HTRF certified microplate reader. For more information about set-up and compatible HTRF® readers,The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READERThis makes the PHERAstar ®FSX the fastest laser-based TRF reader on the market: 8 seconds for a 96-well plate. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. Incubate 4h at RT. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. For more information about HTRF. 3nm, important when reading at 280 nm for DNA analysis, for more exact results. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. Reagents were dispensed following Cisbio’s ligand binding protocol in 384-well format (white plates). Plate Type and Read Area should be selected based on the microplate used and wells to be read. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Plate 25 μL of cells in. FOR HTRF CERTIFIED READER culture plate to a 384-well small volume white plate. PerkinElmer. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. 0. For more information about HTRF. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . The ratio between control lysate signal / non-specific signal should be greater than 2. Dedicated UV-laser excitation. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The lysis buffer 4X is ready-to-use. Step 1 Step 2 Step 3 Step 4 Step 5. Plate Reader Requirement. Read & analyse. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. It features a modular design that allows users to add or change detection cartridges according to their needs. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Catalog No. The Bradford assay was successfully performed on the BMG LABTECH microplate reader. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. FOR HTRF CERTIFIED READER. VICTOR Nivo Multimode Plate Reader. As shown in Fig. HTRF-specific. Incubate 4h at RT. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Dual monochromators for assay flexibility With. Lysates are then transferred to the assay plate for the detection of Total Cofilin by HTRF® reagents. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. HTRF is compatible with a large range of available microplate readers. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. * For HTRF microplate recommendations, please visit . For more information about set-up and compatible HTRF ® readers, please visit our website at: ® pre-mixed antibodies TWO- PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells . ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Simply place the module into one of the five module bays and read the assay. best fluorescence polarization plate reader on the market. 5 μL of Binding buffer 2X. No washing steps are required. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. Use simpler, faster protocols. Plate 25 μL of cells in. Remove the plate seal and read on an HTRF compatible reader. overnight at 4 C [Prostaglandin E2] pg/ml. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. The assay is then ready to be read on an HTRF compatible or certified microplate reader. 96-well culture plate• HTRF®-Certified Reader. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Smallest and most affordable multimode plate reader in the industry. SERIAL DILUTIONS . The controls are equivalent to the upper and lower limit of. Read & analyse. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. This protocol, HTS designed, allows miniaturization while. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Plate 25 μL of cells in. Results for HTRF Reader Control Kit with black and white microplates. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Discover our portfolio of multimode plate readers and radiometric detection solutions, complemented by assays, reagents and consumables which together enable you to increase your lab’s. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. For more information about HTRF. The donor and acceptor can be used to label a variety of biomolecules, for applications. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. EnSight ® offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED READER. It is used as a positive control for Gi applications ( biological models validation,FOR HTRF CERTIFIED READER. Microplate readerFluorescence microplate readers usually contain a light source, an optical system for the selection of excitation light (i. For more information about HTRF ®Seal the plate and incubate for Overnight. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersHTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. DLReady™ certified luminometer plate readers. FlexStation® 3 Multi-Mode Microplate Reader. 96-well culture plate 384SV detection plate- Add . FOR HTRF CERTIFIED READER. Revvity. Evaluation of the performance of the Spark™ multimode reader for far red fluorescence emission. FOR HTRF CERTIFIED READER. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. For more information about HTRF ®The LUMIstar® Omega is BMG LABTECH's powerful dedicated luminescence microplate reader, providing highly sensitive flash and glow assay readings. 4. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Each well requires 10 μL of 4X lysis buffer /well. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READER. Make sure the setup for Tb Cryptate is used. It can also be further streamlinedFOR HTRF CERTIFIED READER. Make sure the plate reader is turnedon and then open the MikroWin software on the computer. Download Setup. After defining the objectives and their locations in Gen5: 1. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:320/40 HTRF Eu cryptate 665/7uv Wide Filter 0. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. * For HTRF microplate recommendations, please visit . FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. Read & analyse. Specialized filters allow for IR fluorescence. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Lysates are then transferred to the assay plate for the detection of Phospho-VASP (Ser239) by HTRF. Read & analyse. Plate 25 μL of cells in. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. FOR HTRF CERTIFIED READER. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. TO PREPARE TISSUE SAMPLES: Please refer to the Technical Note “Optimize Your HTRF® Cell Signaling Assays on Tissues” onHow to use Synergy microplate readers for HTRF detection, a versatile technology for drug discovery and life science research. STANDARD . For more information about HTRF. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. For more. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. HTRF is compatible with a large range of available microplate readers. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Your lab might even have one already. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. FOR HTRF CERTIFIED READER. and TR-FRET, for example to detect PerkinElmer’s DELFIA, HTRF and LANCE assay technologies Time resolved fluorescence certified for use with HTRF. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF® 96-well low volume plate TECHNOLOGY ALL THE BENEFITS OF HTRF WITH THE EASE OF USE OF AN ELISA PLATE!. HTRF-specific. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. 5 EXAMPLE OF MAP PLATE . Configure your microplate reader. Incubate 4h at RT. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. For more information about HTRF. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates to a low volume detection plate (either HTRF 384-lv or 96-lv plate) before the addition of HTRF Phospho-Rb (Ser780) detection reagents. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. 1. 96-well culture plateFOR HTRF CERTIFIED READER. According to the manufacturers protocol, this protein assay is linear in the range of 0. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. + 4 µL HTRF ® pre-mixed antibodies . 47 seconds for a 1536-well plate. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Incubate overnight at RT. For a list of HTRF-compatible readers and set-up recommendations, please visit . These plates can be read on all HTRF certified readers, thus making their implementation in. CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. 25,000 human Jurkat cells were plated in 96 well plates. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed TNFα Antibodies ADD READ ANALYSE Incubate 2 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. We would like to show you a description here but the site won’t allow us. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Incubate overnight at RT. For more information. For more information about HTRF. 8 µL cells. Dedicated UV-laser excitation. HTRF (Homogeneous Time Resolved Fluorescence) is a no-wash technology. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. HTRF is compatible with a large range of available microplate readers. 5 hours before 3 more washing steps.